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KMID : 1134820160450070938
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Journal of the Korean Society of Food Science and Nutrition
2016 Volume.45 No. 7 p.938 ~ p.947
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Neuronal Cell Protective Effect of Dendropanax morbifera Extract against High Glucose-Induced Oxidative Stress
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Kim Jong-Min
Park Seon-Kyeong
Guo Tian Jiao
Kang Jin-Yong
Ha Jeong-Su
Lee Doo-Sang
Kwon Oh-Jun
Lee Uk
Heo Ho-Jin
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Abstract
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Antioxidant activities and neuroprotective effects of ethyl acetate fraction from Dendropanax morbifera (EFDM) against high glucose-induced oxidative stress and neurotoxicity were investigated to confirm their physiological activities. An 80% ethanolic extract of D. morbifera showed the highest contents of total phenolic compounds as well as 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and 1,1-diphenyl-2-picrylhydrazyl radical scavenging activities. The extract was fractionated using several solvents, and the ethyl acetate fraction showed the highest activities in ferric reducing/antioxidant power and malondialdehyde inhibitory assays. To evaluate the neuroprotective effect based on antioxidant activities, cell viability was assessed using PC12 and MC-ⅨC cells in H2O2- and high glucose-induced cytotoxic assays, respectively. EFDM evidently showed neuroprotective effects in all cells (neuron-like PC12 cells and human brain-originated neuroblastoma MC-ⅨC cells). Inhibitory effect of the extract on acetylcholinesterase (AChE) as an acetylcholine-hydrolyzing enzyme was performed to examine the effect on cognitive function. EFDM presented an AChE inhibitory effect. Finally, high-performance liquid chromatography analysis showed that the major phenolic compound of EFDM is probably a rutin.
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KEYWORD
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Dendropanax morbifera, antioxidant, oxidative stress, high glucose, neuroprotective effect
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